sino biological catalog  (Sino Biological)

Journal: Science Advances

Article Title: Conserved sites on the influenza H1 and H3 hemagglutinin recognized by human antibodies

doi: 10.1126/sciadv.adu9140

Figure Lengend Snippet: ( A ) Diversity of influenza A HA. Phylogenetic tree of IAV HAs (left). Timeline of influenza H1N1 and H3N2 circulation; symbols represent strains used in this study (middle). Pairwise identity of HAs from viruses used in this study mapped onto structures of HA [Protein Data Bank (PDB) 3LZG and 4FNK] (right). ( B ) Neutralization potencies determined by microneutralization for down-selected antibodies against a panel of IAVs. Strains are indicated as in (A). H1N1 strains: A/WSN/1933, A/USSR/90/1977, A/Memphis/4/1987, A/Massachusetts/1/1990, A/Beijing/262/1995, A/New Caledonia/20/1999, A/Solomon Islands/3/2006, A/New York/08-1326/2008, A/California/07/2009, A/Michigan/45/2015, and A/Sydney/5/2021. H3N2 strains: A/Aichi/2/1968, A/Bilthoven/1761/1976, A/Beijing/353/1989, A/Johannesburg/33/1994, A/Brisbane/8/1996, A/Fujian/411/2002, A/Wisconsin/67/2005, A/Perth/16/2009, A/Switzerland/9715293/2013, and A/Kansas/14/2017. Neutralization assays were performed in technical duplicate. N.N., non-neutralizing. ( C ) ELISA binding reactivity at 10 or 1 μg/ml to A/Swine/Henan/SN13/2018 or A/Canine/Hainan/79/2019 (left) and BLI-based antibody competition with the indicated antibodies (right).

Article Snippet: Briefly, His-tagged H1N1 A/California/04/2009 full-length trimer Y97F (Sino Biological, catalog no. 11055-V08B1) was mixed in 4:1 molar ratios with SA-allophycocyanin (APC; Invitrogen) and SA-phycoerythrin (PE; Invitrogen) whereas A/Darwin/6/2021 full-length soluble trimer and A/Hong Kong/1/1968 head trimer (see below) were mixed in 4:1 molar ratios with SA-PE (Invitrogen) and SA-APC (Invitrogen), respectively, and were incubated for 20 min on ice.

Techniques: Neutralization, Enzyme-linked Immunosorbent Assay, Binding Assay

Journal: Cell Reports Medicine

Article Title: Modulation of lipid nanoparticle-formulated plasmid DNA drives innate immune activation promoting adaptive immunity

doi: 10.1016/j.xcrm.2025.102035

Figure Lengend Snippet: Initial immune characterization of HA DNA-LNP formulations and immunogenicity (A) Schematic of DNA-LNP N/P ratios and immunization regimen. (B–D) Biophysical characterization of DNA-LNPs at different N/P ratios. (B) Particle size; (C) polydispersity index (PDI); (D) zeta potential. (E) Representative fluorescence-activated cell sorting (FACS) plots of GC B cells. (F) Bar plots quantifying frequency of GC B cells. (G) Frequency of CA09 HA-specific GC B cells. (H) Frequency of activated Tfh cells. (I) IFNγ ELISpot of splenocytes. (J) Representative TEM images of HA DNA-LNP (top) and HA mRNA-LNP (bottom). Scale bar 100 nm (K and L) Fold change cytokine induction in DLNs at 4 h (K) and 24 h (L) after immunization quantified using Luminex. (M and N) ELISpot assay measuring IFNα (M) and IFNγ (N) 20 h after stimulation of splenocytes ex vivo with DNA-LNP, plasmid DNA, or DNA-LNP in the presence of chemical inhibitors to the indicated DNA sensors. (O) Schematic of relevant pathways implicated in DNA-LNP sensing. Dots represent individual animals; n = 8–9 (E–H), n = 5 (I, K, and L), or n = 3–4 animals per group (L and M); data pooled or representative from two independent experiments (E–H, M, and N) or from one independent experiment (I–L). Plots show mean with SD (B–D and I) or geometric mean with geometric SD (F–H and K–N). Unpaired one-way ANOVA adjusted for multiple comparisons with Bonferroni corrections was used to compare groups (F–H, K, and L) or compared to DNA-LNP control (M and N). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: B cell probes were generated via direct conjugation of fluorochromes to recombinant influenza virus CA09 HA (Cat# 11055-V08B1, Sino Biologicals).

Techniques: Zeta Potential Analyzer, Fluorescence, FACS, Enzyme-linked Immunospot, Luminex, Ex Vivo, Plasmid Preparation, Control

Journal: Cell Reports Medicine

Article Title: Modulation of lipid nanoparticle-formulated plasmid DNA drives innate immune activation promoting adaptive immunity

doi: 10.1016/j.xcrm.2025.102035

Figure Lengend Snippet: HA DNA-LNP induces robust GC and serum responses Mice were immunized with HA DNA-LNP (2 μg), HA mRNA-LNP (2 μg), or adjuvanted HA protein (1 μg). GC responses were assessed in the DLNs 14 days post immunization and serum responses longitudinally. (A) Representative FACS plots of activated Tfh cells. (B and C) Bar plots show quantification of frequency (B) and numbers (C) of activated Tfh cells. (D) Representative FACS plots of total GC B cells. (E and F) Bar plots show quantification of frequency (E) and numbers (F) of total GC B cells. (G) Representative FACS plots of CA09 HA-specific GC B cells. (H and I) Bar plots show frequency (H) and numbers (I) of CA09 HA-specific GC B cells. (J) Area under the curve (AUC) of total A/California/04/2009 HA-specific serum IgG ELISA data. (K) Serum endpoint titers at week 8 to various H1N1 HAs. (L) HAI titers at week 8 to A/California/07/2009 X-179A. (M and N) AUC of serum binding antibodies to A/Guangdong-Maonan/SWL1536/2019 HA (M) and A/Victoria/4897/2022 HA (N). (O and P) HAI titers to A/Netherlands/602/2009 (O) and A/New York City/PV63249/2022 (P). Dots represent individual animals (B, C, E, F, H, I, K, and L); n = 9–10 animals per group; data pooled from two independent experiments. Plots show geometric mean with geometric SD. Unpaired one-way ANOVA adjusted for multiple comparisons with Bonferroni corrections was used to compare groups (A–I) or active immunization groups (K). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: B cell probes were generated via direct conjugation of fluorochromes to recombinant influenza virus CA09 HA (Cat# 11055-V08B1, Sino Biologicals).

Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay

Journal: Cell Reports Medicine

Article Title: Modulation of lipid nanoparticle-formulated plasmid DNA drives innate immune activation promoting adaptive immunity

doi: 10.1016/j.xcrm.2025.102035

Figure Lengend Snippet: HA DNA-LNP induces potent memory responses in mice and rabbits (A) Schematic of mouse immunization regimen. (B) IFNγ-secreting cells in splenocytes by ELISpot. (C) IFNγ-secreting effector CD8 + T cells by flow cytometry. (D) CA09 HA-specific ASC responses in bone marrow by ELISpot. (E) Representative FACS plot of CA09 HA-specific MBCs. (F and G) Bar plots show frequency (F) and numbers (G) of CA09 HA-specific MBCs. (H) Schematic of rabbit immunization regimen. (I–K) IFNγ ELISpot on peripheral blood mononuclear cells (PBMCs) at day 42 (I), day 105 (J), and day 202 (K). (L) AUC of total A/California/04/2009 HA-specific serum IgG ELISA data. (M and N) HAI titers to A/Netherlands/602/2009 (M) and A/New York City/PV63249/2022 (N). Dots represent individual animals (C, D, F, and G); n = 9–10 animals per group (B–D, F, and G), n = 5 animals per group (I–N); data pooled from two independent experiments. Plots show mean with SD (B and I–K) or geometric mean with geometric SD (C, D, F, G, and L–N). Unpaired one-way or two-way ANOVA adjusted for multiple comparisons with Bonferroni corrections was used to compare groups. ANOVA was performed at the final time point for (L–N). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: B cell probes were generated via direct conjugation of fluorochromes to recombinant influenza virus CA09 HA (Cat# 11055-V08B1, Sino Biologicals).

Techniques: Enzyme-linked Immunospot, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Cell Reports Medicine

Article Title: Modulation of lipid nanoparticle-formulated plasmid DNA drives innate immune activation promoting adaptive immunity

doi: 10.1016/j.xcrm.2025.102035

Figure Lengend Snippet:

Article Snippet: B cell probes were generated via direct conjugation of fluorochromes to recombinant influenza virus CA09 HA (Cat# 11055-V08B1, Sino Biologicals).

Techniques: Virus, Recombinant, Lysis, Reporter Gene Assay, Cell Stimulation, Electron Microscopy, Luminex, Enzyme-linked Immunospot, Luciferase, Plasmid Preparation, Software, Synthesized

Journal: bioRxiv

Article Title: Conserved sites on the influenza H1 and H3 hemagglutinin recognized by human antibodies

doi: 10.1101/2024.10.22.619298

Figure Lengend Snippet: ( A ) Diversity of influenza A HA. Phylogenetic tree of influenza A virus HAs (left). Timeline of influenza H1N1 and H3N2 circulation; symbols represent strains used in this study (middle). Pairwise identity of HAs from viruses used in this study mapped onto structures of HA (PDB: 3LZG and 4FNK) (right). ( B ) Neutralization potencies determined by microneutralization for down-selected antibodies against a panel of influenza A viruses. Strains are indicated as in A . H1N1 strains: A/WSN/1933, A/USSR/90/1977, A/Memphis/4/1987, A/Massachusetts/1/1990, A/Beijing/262/1995, A/New Caledonia/20/1999, A/Solomon Islands/3/2006, A/New York/08-1326/2008, A/California/07/2009, A/Michigan/45/2015, A/Sydney/5/2021. H3N2 strains: A/Aichi/2/1968, A/Bilthoven/1761/1976, A/Beijing/353/1989, A/Johannesburg/33/1994, A/Brisbane/8/1996, A/Fujian/411/2002, A/Wisconsin/67/2005, A/Perth/16/2009, A/Switzerland/9715293/2013, A/Kansas/14/2017. ( C ) ELISA binding reactivity at 10 or 1 µg/ml to A/Swine/Henan/SN13/2018 or A/Canine/Hainan/79/2019 (left) and BLI-based antibody competition with the indicated antibodies (right).

Article Snippet: Briefly, His-tagged H1N1 A/California/04/2009 full length trimer Y97F (Sino Biological Cat # 11055-V08B1) was mixed in 4:1 molar ratios with SA-Allophycocyanin (APC; Invitrogen) and SA phycoerythrin (PE; Invitrogen) while A/Darwin/6/2021 full-length soluble trimer and A/Hong Kong/1/1968 head trimer (see below) were mixed in 4:1 molar ratios with SA phycoerythrin (PE; Invitrogen) and SA-Allophycocyanin (APC; Invitrogen), respectively, and were incubated for 20 min on ice.

Techniques: Virus, Neutralization, Enzyme-linked Immunosorbent Assay, Binding Assay